Super production of alcohol using plant gene in yeast
National Institute of Plant Genome Research-New Delhi published a paper with title “Expression of AtMed15 of Arabidopsis in yeast causes flocculation and increases ethanol production in yeast culture” in NATURE Scientific reports on 16 of June this year. As per the study the yeast expressing a mediator complex protein from Arabidopsis called AtMed15 boosted the alcohol production.
A multiprotein complex such as Med15 required in reading of class II genes, was initially found in yeast, and afterward portrayed in numerous metazoans uncovering a striking basic preservation of the complex. Bioinformatics examination of all the Mediator subunits and biochemical exclusion of Mediator complex from Arabidopsis proposed that plant genomes additionally code for Med15 ortholog hence named as AtMed15. After discovering the gene AtMed15, the team wanted to test its functionality in yeast, as AtMed15 known to play role similar to ScGAL11 (Subunit of the RNA polymerase II mediator complex).
During the experimentations it was observed that AtMed15 presence in yeast upturns adhesiveness of cells by triggering flocculation. Factors like pH and temperature had no effect on boosted flocculation, however, EDTA and CaCl2 addition shown to modulate the mass of flocculation in yeast with AtMed15 expression. The molecular events responsible for the enhanced flocculation of yeast with AtMed15 was seem to be due to increased levels of Flo gene family (Flo1, Flo5, Muc1 and Aga1).
Yeast scientifically known as S. cerevisiae and, is utilised for maturing of sugars into liquor has a long relationship with the sustenance and mixed drink commercial ventures and creation of bio-ethanol. Yeast strains with AtMed15 shown have increased flocculation, which means increase fermentation. The one way to measure the fermentation is to measure the alcohol content of the yeast cultures and can be further enhanced by adding sugars. While applying similar stagey to yeast with AtMed15 the group found that the AtMed15 have greater fermentation capacity, increased alcohol production and adding sugars robustly increased the same. This procedure for expanded ethanol generation can be misused for use in bio-ethanol creation, and the bottling works and drinks businesses.
Dahiya, P. et al. Expression of AtMed15 of Arabidopsis in yeast causes flocculation and increases ethanol production in yeast culture. Sci. Rep. 6, 27967; doi: 10.1038/srep27967 (2016).